Journal article
S. Kim, Thavasyappan Thambi, V. H. Giang Phan, D. S. Lee
Carbohydrate Polymers, 2020
Carbohydrate Polymers, 2020
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APA
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Kim, S., Thambi, T., Phan, V. H. G., & Lee, D. S. (2020). Modularly engineered alginate bioconjugate hydrogel as biocompatible injectable scaffold for in situ biomineralization. Carbohydrate Polymers.
Chicago/Turabian
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Kim, S., Thavasyappan Thambi, V. H. Giang Phan, and D. S. Lee. “Modularly Engineered Alginate Bioconjugate Hydrogel as Biocompatible Injectable Scaffold for in Situ Biomineralization.” Carbohydrate Polymers (2020).
MLA
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Kim, S., et al. “Modularly Engineered Alginate Bioconjugate Hydrogel as Biocompatible Injectable Scaffold for in Situ Biomineralization.” Carbohydrate Polymers, 2020.
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@article{s2020a,
title = {Modularly engineered alginate bioconjugate hydrogel as biocompatible injectable scaffold for in situ biomineralization.},
year = {2020},
journal = {Carbohydrate Polymers},
author = {Kim, S. and Thambi, Thavasyappan and Phan, V. H. Giang and Lee, D. S.}
}
Abstract
In the present study, a type of bioconjugate was synthesized by post modification of alginate by conjugating temperature-responsive poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide) and O-phosphorylethanolamine as phosphorylation functional groups. Freely flowing bioconjugate sols at low temperature can transform to stable viscoelastic gels at the physiological temperature (37 °C). Subcutaneous administration of temperature-responsive bioconjugate sols into the dorsal region of Sprague-Dawley rats formed in situ hydrogel. in situ formation of bioconjugate gels in stimulated body fluids at 37 °C showed nucleation and hydroxyapatite mineral growth. Furthermore, hydroxyapatite growth was also found in in vivo gels, which suggested the potential of alginate-based bioconjugate gels as a scaffold for bone engineering. Bone morphogenetic protein 2 (BMP-2)-loaded bioconjugate formed stable gel in vivo, and demonstrated sustained release. BMP-2-loaded bioconjugates exhibited in situ biomineralization in vivo. These results imply that the in situ formation of injectable biomimetic materials has potential for bone tissue engineering applications.